DNACROBATICS & BUTTERFLY EVO-DEVO
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REARING AND HANDLING

Injection of Butterfly Eggs

by: Amruta Tendolkar

Butterfly eggs are collected on the host plant leaf. The eggs are moved to an injection petri dish that is lined with a double-sided sticky tape. The eggs are positioned in the correct orientation with the micropyle facing up and gently pressed down so that they don’t move during injections. Guide RNA against the target gene and Cas9 protein is injected into the egg through the micropyle.
More information: 
  • Martin A, Wolcott NS, O’Connell LA. Bringing immersive science to undergraduate laboratory courses using CRISPR gene knockouts in frogs and butterflies. Journal of Experimental Biology. 2019 (accepted article, in press). [link] [pdf]
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Picture

CONTACT

Dr. Arnaud Martin
The George Washington University
Department of Biological Sciences
800 22nd St NW (SEH6000)
Washington, DC 20052
EMAIL ME
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
  • Home
  • Butterflies
    • Rearing and Handling
    • Findings
  • Moth
    • Getting Started
    • Rearing
    • Life Cycle & Sexing
    • Crossing
    • Molecular Biology
    • Resources
  • Resources
    • Protocols
    • Publications
    • GEPHEBASE
  • Bionautes
    • Join Us
  • Teaching