Injection of Butterfly Eggs
by: Amruta Tendolkar
Butterfly eggs are collected on the host plant leaf. The eggs are moved to an injection petri dish that is lined with a double-sided sticky tape. The eggs are positioned in the correct orientation with the micropyle facing up and gently pressed down so that they don’t move during injections. Guide RNA against the target gene and Cas9 protein is injected into the egg through the micropyle.