Photo credit: Flickr user Caramosca (CC BY-NC 2.0) ; https://flic.kr/p/2bLPu4q
Genotyping (white gene)
One leg of a frozen Plodia individual is isolated with forceps and submerged into DNARelease solution in a PCR vial. In between samples, forceps are rinsed in 5% bleach, deionized water, and 70% Ethanol. The tissue is then heated at 98'C for 2 minutes and 1 ul of the sample is added into prepared PCR mix to amplify the white gene. Half of the PCR product is then digested with NlaIV enzyme and run on gel. Heterozygous and homozygous samples will show up as four bands and two bands, respectively.